Coding

Part:BBa_K2619011:Design

Designed by: Miaoyan Li   Group: iGEM18_XJTLU-CHINA   (2018-10-16)


HSP70/Heat shock protein 70 (Homo sapiens)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 362
    Illegal BglII site found at 1286
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 447
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 337
    Illegal SapI.rc site found at 1656


Design Notes

We added KOZAK sequence to enhance expression of HSP70 in target neuron.


Source

It comes from genomic sequence of Homo sapiens.

References